|
KMID : 0624620100430040284
|
|
BMB Reports
2010 Volume.43 No. 4 p.284 ~ p.290
|
|
|
Expression and characterization of RNA-dependent RNA polymerase of Ectropis obliqua virus
|
|
Lin Mei-Juan
Ye Shan
Xiong Yi
Cai Da-Wei
Zhang Jia-Min
Hu Yuan-Yang
|
|
|
Abstract
|
|
|
|
Replication of positive-strand RNA virus is mediated by a virus- encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Ectropis obliqua virus (EoV), a newly identified insect virus belonging to the family Iflaviradae, we expressed the RNA polymerase domain in Escherichia coli and purified it on a Ni-chelating HisTrap affinity column. It is demonstrated that EoV RdRp initiated RNA synthesis in a primerand poly (A)-dependent manner in vitro. Furthermore, the effect of primer concentration, temperature, metal ions (Mg2+, Mn2+, and K+) on enzymatic activity were determined. Our study represented a first step towards understanding the mechanism of EoV replication.
|
|
|
KEYWORD
|
|
|
Ectropis obliqua virus, Iflavirus, Primer-dependent, RNAdependent RNA polymerase, RNA synthesis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|